10:00 am – 8:00 pm, 9:00 pm – 11:20 pm
Ph KD BXC-F6 further imaging
STORM
- sample chamber contaminated by Draq5 — can’t see dye switching
CycA
- cytoplasmic antibody stain still present after hybridization
Sample prep
- preparing new sample with BXC-P1 S1 and F6 in P3-750
- hybridized and staining, ~10:30 am
Ph antibody imaging
- rinsing out O/N primary stain.
- brief blocking
- incubate in secondary antibody (rb-488).
- some nuclei actually show good staining (some don’t). Maybe 1%-tween with block wasn’t sufficient permiabilization.
- repeat with Triton-X 10 min treatment, running Ph-WT and Ph-KD in perfect parallel
QPCR
- 9 primer-pairs + 8 samples
- 12.5 uL 2x Master, 6.25 uL ddH2O, 1.25 uL EvaGreen, 2.5 uL primer mix, 2.5 uL DNA
- 1 mL master, 500 uL ddH2O, 100 uL Eva Green (500 + 250 + 50);
- primers and cDNAs as labeled here:
Presentations
- working on lab meeting presentation
- working on figures for review to discuss at XZ meeting