Tuesday 09/08/15

9:00 am – 8:00 pm

Revising manuscript

  • New versions of all the main figures and all the extended data figures now started.
  • Figure items: Still need to do
    • deconvolution of conventional images
    • add anti-aliased full size conventional images
    • analysis of Pc KD domains, add to Ph-figure
  • Text things to do
    • revise captions for all figures
    • revise letter, match all figure panel labels
    • add images of figures into letter
    • add methods section for PcG KD and quantification
    • add methods section for NextGen sequencing

Prep

  • wrote new script for designing primers and ensuring different regions are chosen (built on primer3 and NCBI live sequence retrival).
    • script is in ForReviewers folder of chromatin git project
    • saves images
      PrimerDesign
    • green is QPCR primers, red is masked RNAi region. units are in bp
  • ordered new ph-d primers for ‘same day delivery’ (ETA Thursday, how is that same-day, it’s Tuesday still. And these cost 13 each + 20 shipping instead of 3 and free shipping…)
  • ordered more backup ph-d, ph-p and Pc primers

Communication

  • NF inquire about paper
  • discuss recent Fraser Luscombe and Elderkin paper.
  • XZ will get replies back this week.
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