9:00 am – 7:30 pm
Library building code
- fixed bugs in library design — can’t call TRDesigner with parallel pool with large off-target libraries.
- it takes a ton of time and a ton of memory to replicate these large OTTables
- this is blazingly fast in non-parallel mode.
- No need to use parallelization in building OTTables — launching the parallel matlab cores is slow, uses more memory, and doesn’t speed things up for this code (at least on the scale of my current library design).
- worked out primer building
- don’t actually need lots of index primers, part of the beauty of the new design is to move away from having tons of individually indexed regions.
- requested readout probes sequences from SW, stick with 30mers, these are working well.
Updated scripts
- AssembleChrLib7_151103
- S151103_PlotCutSitesAndChIPdata
- (finished creating coverage vectors for all ChIP-seq data).
Presentations
- seminar on interviews (see notes)
- practicing talk for DF interview tomorrow.