Maggert lab, (Texas A&M)
Ctcf regulates Erna synthesis in nucleuolos
spatial and temporal dynamics of homologous recomb reapir. (Karpen lab)
double strand breaks in heterochromatin/ rpt dna.
Double strand breaks peak earlier in heterochromatin.
after irradiation expansion of hp1a
Margarete Heck (Edinburgh)
Invadolysin
fuzzy, hypercondensed chromosomes during mitosis.
Localizes to leading edge of ligating cells. Ubiquitnaled H2b accumulates in cytoplasm. Why is h3k4 expressed at higher levels?
H2b deubiquitanase.
Alexey Soshnev (Pamela Geyer lab, Iowa)
Su(Hw) insulator fxn and female sterility. Genetically separable.
Jack Bateman (Bowdoin) Enhancer action / Transvection
somatic homolog pairing/ transvection
yellow enhancers can act in trans. When yellow gene is dead but promoter is okay no transvection.
enhancerless and promoterless pair in gmr. enhancer.
Some genomic loci don’t support transvection.
trans expression weaker than Cis, strong trans sites are strong cis sites.
gmr driving lacs both get turned on by a single cell. Every cell gets cis, a few cells also get trans.
Allison Schaaf (Brian DeDecker lab, UC Bolder)
H3.3 partial lethality, transcriptions irregulatory. Affects Notch expression (m3 ) notch H3.3 doubles viability increases to 80% from 50% (12%) if no maternal.
claim 3.3a activate su(H). Adding su(H) w/ Gal4. Non-replicating variant is now regulatory.
Yuri Schwartz, Vincenzo Pirrotta (Rutgers) PHO and PHOL
PHOL binds to different sites than PHO more promoters less PREs. Genetically somewhat redundant. Compete for sfmbt which binds PREs first.
YY1 probably not human polycomb group.
Chiat Lim / Richard Kelley (Baylor)
Dosage compensation. MSL complex, redundant rox genes are required targets. Very diff in size sequence and tx control. Rox 1 expression dependent on early mRNA burst.