PCR cloning for tup-exon thus-exon and pnr-exon probes (respectively):
- Gel extracting and ligating: tup 30ng/uL, pnr 15 ng/uL, ths 15 ng/uL
- Ligate at 10 uL pnr and ths and 5uL tup to 2 uL pGEM, 20 uL rxn. 3 uL positive control DNA.
- Transform competent cells.