10:15 A – 5:15 P, 11:00 P -12:30 A
- Flip collection cages: MP06, MP01, MP02, MP10; sna[18], MP08; sna[D], 10:15A. Freeze MP09 Hz.
- Flip collection cages MP06, MP01, MP20, MP10; sna[18], 12:15 P.
- Check plates: colonies look good for all cloning: ths-e, pnr-e, tup-e. Moved to 4C. Pick colonies tonight.
- Set up PCR for controls using yellow-probe fragments.
- Finish ON confocal run, 10 embryos by 12:00P, MP02_hb_y_b. Copy data.
- Running analysis on MP01_hb_y_f data. Memory errors every second embryo.
- Add more Fly collaborations to JF collaboration proposal. Troubleshooting font conversion with TeX to PDF with help of JF tech.
- Flip stock bottles: MP01, MP06, MP10, 6x bcd, 2.8-snaD.
- Fix embryos, 2:30 P: MP01, MP06, MP10-sna[18], MP02.
- Running gel to test yellow short probes 1 and 2.
- Quick check: half life 7 min, production 12/min, steady state 120.
- Reply to Mike re: comments on progress report.
- Pick colonies,