Probe template making

b-full, sog (R30), sna (R16), y(i), y(f), tupA, pnrA, thsA

Most lanes too much loading DNA (amplification efficiency looks inversely proportional to the amount of DNA).

Gel extract: snail is too low: 3 ng/uL, others are 14-70 ng/uL.



Gel extract concentrations:
pnr: 18, ths: 24, tup: 69, y(full): 21, y(intron): 43, sog(R30): 14
probes in Lin. Probe. Templates box.


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