Sunday 05/22/11

1:30P-7:10P, 8:20P-10:30P

  • Collect virgin Gal4-bcd-3utr
  • mount slides from in situs
  • Remove Tuxera.  Install G-RAID and convert to NTFS file format.
  • Sequences (with m13R):
    inv A: +/+/gene is +, T7.   inv B +/-/+, SP6.
    E(Pc) A +/+/+ T7.  E(Pc) B +/-/+ SP6
    en A +/-/- T7.  en B wrong gene.
    tou A +/-/- T7. tou B +/-/- T7.
  • PCR linearize: invA, E(Pc)A, touA, enA
  • flip bottle stock
  • collect virgins
  • cross UAS-twi males x Gal4-bcd-3-UTR.
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