Planning chromatin staining
- inv expression pattern: maternal, broad, late seg-polarity gene.
- en expression pattern: early seg-polarity gene, front on first
- tou expression pattern. Maternal and ubiquitous. Yay.
- E(Pc) expression pattern. ‘faint ubiquitous’. Hope this works.
5′ probe from tou may be too mobile. Currently using 1.3 kb 3′ end probe, about 2.5 kb upstream of the transcription termination site.
E(Pc) big gene lots of coding sequence, locus exon dense. Only good intron is 1kb first intron which is inside the first UTR. 7kb of coding sequence in an 11 kb gene.
