Progress Report
Top Findings:
1. Effect of bcd does on hb expression:
6x bcd have peak hb levels similar to 2x bcd, 1x bcd are close but a little less hb and more variable between embryos.
2. hb-BAC minus shadow produces substantially less yellow-reporter transcript than control BACs. [Also more variable].
3. sna-BAC minus shadow accumulates substantially less sna mRNA in the deletion background (1/3rd or less than edongenous levels). Also more variable.
Goals:
1. mRNA counting in snail rescues with primary deleted (need more images)
2. mRNA counting in snail rescues with unmodified BAC (need more images)
3. Total mRNA in paused / unpaused genes?
I have just made new exonic probes for sog, ths, tup, and pnr. If they are working well I can do the total mRNA comparisons for each of these (and Jacques/Mounia can do some of these will yellow and their promoter swaps).