10:30 am — 5:50 pm, 8:00 pm – 10:00 pm
- Starting protein stain to test antibodies, sectioning, and STORM dyes.
- Santa Cruz Bicoid antibody at 1:500, hb antibody at 1:500 with blocking peptide at 1:500. Incubating at RT 1:30P – 3:30
- Reschedule Meeting with DePace group for Nov 9th.
- Ordering fly fixing supplies
- Get added to STORM 2 calendar
- Get added to Primer ordering PO
- Incubate secondary at RT 2 hrs 15 min. – dk anti-rabbit 405-647 at 2:0.9(?)
- Rinsing secondary
- separate some embryos for direct viewing in PBT