9:45 A – 10:45 P
- Embryos for in situ into hybe. New hybe soln in door in -20C freezer.
- wash out primary antibody for antibody stains
- flip fly stocks
- make new top and side access vacuum embryo chambers
- snaD line not look right (red eyes straight wings) . Get resent?
- Draft snail paper sent to ML
- STORM 2 imaging. dyes seem to bleach quickly — low labeling efficiency maybe? Try to image same slide on Zeiss700 tonight.
- Mixed up soln of methylparaben in ethanol to saturation ~5 mL powder in 50 mL ethanol.
- in situ probes in hybe at 55C in shaker at 700 rpm — keeps in suspension.
- Working on GPU programming code — write script to break image into sections to avoid memory crash error.
- Multi-emitter fitting on Nmax = 2 does give sharper image. Even for 32 pixel images Nmax >2 => crash. At Nmax = 2 64 pixel works fine. Try whole image?
- Out of plastic tubing to make appropriate vacuum waste containers. Need to wait till tomorrow to do fixation in tube. Switch to classic eppendorf approach.
- No DAPI to mount bcd hb embryos with. Wait till tomorrow :(.