10:00A – 8:40 P, 9:45P – 12:00A
- in situs day 2
- yw in situ in new embryo chamber, day 1
- move antibodies to opaque box in 4C under Storm2/Graham bench.
- use on-deck 2 mL tubes to keep hot-wash solution warm (better than 55C downstairs).
- Zeiss 700 Confocal at 2P-7P
- Troubleshooting: secondaries not clearly documented on last set of embedded embryos. Probably used anti-m 750 to fish out the chicken anti-GFP — explains no signal.
- anti-bcd 647 and anti-laminin 647 (no staining detectable in hb channel) crosslinking at 7:20P
- in situs sna-dig and vnd-exon-bio incubating in primary O/N at 4C
- yw in situs incubating at 55C O/N at 6pm: tup 1A dig (2.9kb) and sog-cDNA bio (5kb)
- antibody stains in 50:50 resin:EtOH ON