9:40 A
- Finish O/N confocal of MP08. run successful. Downloaded data.
- Confocal analysis of last round of embryo stains. Need to add post summarizing results.
- Added Monet to filepath at Github AlistairBoettiger. (laptop communicates as a separate user, AlistairMobile). Cloned Github Image_Analysis on to Monet. Still not sure if repo is initialized / can sync from Monet.
- wt 2 (blue 2) embryos good H3K27, good en-stain, bad E(Pc)-bio 488. Embryos now in PBT after 1hr post fix. Add WGA-488 and postfix again? (Sunday)
- Dostatni uses WGA-633 at 1:500. Test out WGA labels
- Acquired WGA-488 from Colenso (recommends 1:5 or 1:10 dilution).
- New wt embryos in PBT (yellow chambers), ready to test DNA dimer dyes from John and WGA at different concentrations.
- New blue 1 chamber in situs day 1.