Tuesday 11/22/11

10:00 A – 8:00P, 9:00P-10:00P

  • Finishing annotation of revised CheckDotUpDown code to do 3D dotfinding.
  • Running test analysis of MP06 data.
  • New method for finding unique spheres is easier to read but much much slower, need to find some solution to this.
  • Flip wt collection cages at 11:00A
  • H3K27-647, en-555, WGA-488 embryos in ethanol in prep for resin embedding
  • hox probes + sna[D] test probe should have shipped from Levine lab.
  • fixed bug in lsm read thanks to Jacques, (see Jacques git)
  • Flip fly stocks + seed 7 new wt bottles with embryos from collection cages.
  • Flip collection cages, 12p, 1P, 2P, 3P, 4P,
  • set up git repository on Monet.
  • Rewrote find unique dots to dramatically improve association.
  • Working on parameter re-optimization for dot counting.
  • updated master version (switch to master: git checkout master,  get latest changes from my branch: git pull origin Alistair, update master: git push origin master: switch back to development branch: git checkout Alistair)
  • signed up via google to StackOverflow.
  • fixed embryos – 1.5-3hr, 3-4hr, 4-5hr, 6-7hr, 7-8hr.  Should have larger cages.
  • attempting 3D reconstructions in fiji — not working.  Appears to be a graphics card issue, works on Tuck.
  • Select and mount for embedding H3K27-647, en-555, WGA-488 embryos.
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