9:30A -7P, 8P-11:45A
- STORM imaging of H3K4me3, 34 positions
- Rec letters for Madhurima
- Rinse out secondaries on antibody tests, in Hoechst, 1:30P
- rinse out primaries on new in situs, in block, 1:30P
- rinse out primaries on bcd,hb stain, in block, 1:30P
- post-fix in DNA dyes tests to prep for embedding (started 1:30P)
- analyze new MP08 and wt cc13 data. Merged git files from laptop’s modifications over the weekend, resolved conflicts. Now running analysis on new MP05 data with export connected dots and final ID map.
- rabbit anti-H3 works great, ant-H3K27ac looks alright
- in situs and bcd/hb embryos in Hoechst, (rinse, then postfix) 7P
- DNA stains in ethanol after post-fix, ready for resin 7p
- H3 and H3K27ac embryos in PBT ready for post fix 7p
- Freeze fly collection cages
- STORM imaging fails: Hal won’t talk to 405 or 561 laser 🙁
- accepted review for JTB (due Dec 21)