11:30 A – 9:00P, 11:00P – 12:00A
- in situs day 2
- aliquot Heparin and ssDNA to avoid freeze-thaw cycles
- in situ notes: embryos in vial 1 turn yellow, fixation difference?
- 30 min sodium ethoxide treatment for histone-3 slide.
- STORM imaging of histone 3. Using 488 background for alignment. Using switching channel mode instead of quad view.
- in situs in primary antibodies O/N at 4C, (1:400 m a-bio, 1:400 shp a-dig, 1:150 rab a-H3K27). see Embryo_staining