10:30A -8:00P
- in situs day 3 with KE
- working on snail paper figures
- STORM labeling antibodies: see STORM_dyes.
- attempted direct labeling of primary H3K27me3 with 750 and H3K27ac with 647. essentially no dye sticks. Try to repeat with diluted antibodies
- Meeting with NF discuss Polycomb.
- Research proposal sent to dad and NF for feedback.
- 3D imaging of individual nuclei with Matlab and Fiji.
- Day 3 in situs with KE, mount embryos. (20 min SM labeling followed by 10 min wash and quick rinse). Remaining embryos at 4C O/N.