9:45 A – 1:30A
- Flip fly plates, 10A
- lab meeting — planning STORM workshop.
- Fix embryos, 15 min wt fix, 2-5 hrs. 25 min MP08 fix, embryos added to todays in situs.
- STORM analysis to test parameters for S45 data.
- Start in situs for YW 2.5-5.5 embryos and MP08s (up through post-fix)
- STORM imaging of slides: Pc, En slide 2, ubx, H1, H3
- H1 all channels look very weak, all embryos checked all channels show stronger cytoplasmic than nuclear signal. Not sure how this could be given confocal images.