10:00A — 12:20 A
- Flip fly cage, 10:00A
- confocal imaging of H2 (multi histone stains, slide 70) embryos to cut.
- Mark region imaged by confocal using dissecting scope in CBI.
- confocal images of S6, S7, H3, sections marked, thin-sectioned onto slides.
- confocal imaging of PcG during mitosis.
- fixed embryos 2.5-6.5 hrs.
- 10′ minimal fix of embryos 4-7 hrs, stained with PcG antibodies O/N.
- PcG antibodies: used original 1:200 dilutions (3rd round now) added 2.5 uL to each, approx ~1:100 dilution now.