9:40 A – 7:40P, 8:30P – 12:15P
- Setup sample S2 33o nm (4 color) and Psc-hox 330 nm (2 color) to run through Insight.
- Sectioning: 2 slides of 2H and 2 slides of rad21 cut. slides 2H react strongly to cholorform, spread out considerably. rad21 much less evident reaction.
- Check: confocal images of rad21 and 2H do not have the PcG like bodies that appeared in the MP09 slide — doesn’t look like this is an artifact from the rb-647 antibody.
- Insight analysis finished
- running calibration from beads for 647 3D.
- Missed Francis Group meeting
- Rewrote z-analysis script to automatically determine thickness of slice and zmin and zmax by clustering dots in histogram into N clusters where N is increased until the major cluster contains only dots inside the expected range.
- Work out miji and MIJ to call Fiji from within matlab to automatically perform all transforms from the same matlab file.
- Failed repeatedly to change permission on matlab system folders from read only to write. Unable to install java 3D for Fiji maltab. Stupid Windows.
- Projecting 3D data from 3.3 microns of Psc + chromatin imaging.