11:00A – 12:30 A
- Complete and mail DR app.
- Cloning!
- Dilute primers. PCR amplify: Taf1, aTub84b, Neu3 from MP, en from BAC7
- Modencode chromatin tracks failing to load again. Wrote to modencode help.
- Working on drift correction. Wrote drift simulation and drift corrector for arbitrary drift functions. Just need to add interpolator to drift computation.
- Fixed modencode display with help of modencode staff. Selecting ‘reset to defaults’ from the file menu fixes the upload.
- GPU multi running extremely slowly. Probably haven’t normalized counts to photons accurately. Will troubleshoot when computer returns control to user.
- Need DipImage, Visual C++ 2010, and SDK compiler. See here: http://www.mathworks.com/support/compilers/R2011a/win64.html
- Code runs. Code is Much Much faster on smaller images (12×12 pixels) and large numbers of frames.
- Code as sent is just fitting dot regions of interest — it is not designed for a full STORM movie — this part is supposed to be done and segmented in the CPU.
- Wrote to Fang about calling the GPUgaussMLE_z fxn instead of the multiemitter in code that handles the full image.
- PCRs work.
- engrailed from BAC7, Taf1, alpha Tub, Neu3 (3 min elongation not long enough for 5 kb probe most of the time). Negative control (mismatched primers and DNA),
Ladder: (from bottom 500bp, 1kb, 1.5 kb, 2 kb, 3kb etc)
- STORM2 still occassionally loses focus (even with reasonably small step size). Flickering the focus lock on and off still fixes focus.
- Testing antibodies from Hybridoma bank on full fixed embryos. Embryos in antibody at 1:150 O/N. (non-concentrated sera antibodies are at 1:15).