Friday 03/16/12

9:00 A — 9:30P

  • Finish O/N confocal run, save data
  • Projecting data in Matlab
  • Make new and autoclave: 2x YT
  • aliquot IPTG and X-Gal
  • Transform and plate competent cells, 5:30 plates at 37C O/N.  300 uL per plate
  • PCR with 5 min extension for Hox probes
  • PCR with 3.5 min extension for en probes (plan to remake)
  • code mRNA dot finding / and fitting from matlab to call insight for the fits.    Need to automatically write .dax, .inf, and .ini files.  Works.
  • cut 3 slides of Pho K27 at 200 nm.
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