9:45 A -12:20 A
- O/N confocal run failed horribly. Despite warm-up, quick reset of positions, and quick calibration run, devastating z change of focus occurred during run.
- Rinsing out primary antibodies
- GPU multi-fit code still running slow (70 min+ per channel per position).
- GPU single-fit running much faster (3 min per channel per position)
- hybridizing short fix MP09 bcd embryos
- Meeting with Xiaowei (4P-6P)
- Rinsing out hybe
- rpt confocal O/N of 4x sna embryos. Try 10 um offset between center position of 1st embryo and other embryos.