8:50 A — 11:30P
- Finish O/N confocal of MP08.
- For Zeiss710: a 1.7 um offset from center, with 8 um difference between top plane and imaging plane for the first sample only (with other samples all set to top plane) gives good thermal drift compensation.
- STORM WORKSHOP DAY1
- mEOS imaging fails first round — substantial difference in TIRF position due to mEOS filter cube. good TIRF for 647 is past hard TIRF for 561 for EOS.
- Confocal during day of MP08 sample, few more cc13 embryos.
- Running dot-counting analysis of MP08 samples imaged.
- Designing probes for Dfd
- Flip Fly stocks
- look up shRNA hairpin-type and UAS construct. Have several valium22 (second generation short-hairpin, best knockdown efficiency).