Tuesday 04/03/12

10:00 A – 11:00P

  • washing out primary antibodies (after 10 hrs at 4C)
  • maybe should do longer, return to 1 hr RT followed by O/N 4C?
    We’ll see how these ones look.
  • Confocal images of embedded embryos for sectioning today
  • Leica UC7 repair
  • Fix Pc-GFP and en-GFP O/N for check.  Stain with WGA-647 and mount
  • hybing oligo-paint to embryos in 2x SSCT/formamide at 37 O/N (50 pmol in 200 uL)
  • Label RT 3 hrs first round antibodies
  • Label O/N with Dm0 direct labels Cy3B for Hp1a H3 and Bmi Psc samples
  • and O/N secondaries 750 & 647 for PcG histone comparisons
  • sectioning Bcd hb-y(i) sample.  Poorly trimmed, cutting poorly.
  • Bcd hb-y(i) sample far from completely etched after two 40 min etching sessions.  Changed the sodium ethoxide for new batch (From 10/11 stock) for second etching.  Little improvement.
  • Cut 4 slides of en tou E(Pc) H3 at 330 nm.  Cutting very nicely.
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