10:00 A – 11:00P
- washing out primary antibodies (after 10 hrs at 4C)
- maybe should do longer, return to 1 hr RT followed by O/N 4C?
We’ll see how these ones look. - Confocal images of embedded embryos for sectioning today
- Leica UC7 repair
- Fix Pc-GFP and en-GFP O/N for check. Stain with WGA-647 and mount
- hybing oligo-paint to embryos in 2x SSCT/formamide at 37 O/N (50 pmol in 200 uL)
- Label RT 3 hrs first round antibodies
- Label O/N with Dm0 direct labels Cy3B for Hp1a H3 and Bmi Psc samples
- and O/N secondaries 750 & 647 for PcG histone comparisons
- sectioning Bcd hb-y(i) sample. Poorly trimmed, cutting poorly.
- Bcd hb-y(i) sample far from completely etched after two 40 min etching sessions. Changed the sodium ethoxide for new batch (From 10/11 stock) for second etching. Little improvement.
- Cut 4 slides of en tou E(Pc) H3 at 330 nm. Cutting very nicely.