Sunday 04/15/12

4:30 P – 11:00P

  • STORM imaging. HP1-750 slide 1 not fully etched, only some nuclei switch. 1hr 15 min etching.
  • STORM imaging Hp1-750 slide 2 over-etched, 750 switching great, 647 dye almost all lost, very weak by conventional and few switching cycles.  Used fresh cold, anhydrous sodium ethoxide for 2hrs.  Too much.
  • Go for fresh sodium ethoxide for 1hr and check.
  • Cutting new sections of 4H (H3 in 4 colors).
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