10:00A – 8:30P, 9:30-10:30P
- Eileen Group meeting
- invitation to SDB to chair postdoc session and speak in regulatory mechanisms section
- NuGru presentation: Swr1 structural analysis (inserts H2AZ flanking nucleosome depleted regions), for example to protect promoter.
- Finished 3D bead warping code. Using PcG bead-data, 90% of beads aligned to ~50nm in 488 and 561, ~90nm in 750.
- Implemented first complete version of VectorSTORM, create vector images of all molecule locations, corrected for drift and chromatic aberration, and compute intermolecule distances between clusters.
- Francis lab Lit Review A. poor showing for papers.
- Trouble with 750 chromatic alignment — looked good applied to beads, applied to data introduces obvious false shift. Might be due to 512×512 quadview acquisition for beads vs 256×256 acquisition for data.
- Now BeadWarp3D is no longer connecting beads in 561 and 488 correctly. Troubleshooting this…
- Fixed BeadWarp3D (had shortened channel loop for previous troubleshoot). Fixed 750 alignment: need to get rid of scale from BeadWarp. just do everything on the raw positions [0,256].
- MP08s 3 vials, basically all dead adults. Not clear why. hardly any progeny. why is this line so sick?! 2.8 snaD also struggling. MP07 and MP12 rescues look healthy.