10:40 A – 7:00P, 8:45P – 11:45 P
- Flip fly bottles: Pc-GFP. Now growing at 25C
- Flip into single bottles for bottle stock: Pc-shRNA1 and 2, Psc-shRNA1, Bcd-GFP, eve-GFP, en-GFP. Pc-shRNA lines strongly female gender biased.
- Primary stocks at 18C look fine. Give another week or two before flipping
- MP08 line completely dead (strongly male gender biased). Also die as late pupae.
- YW and MP09 bottles at 25C
- interesting article on course on ‘biological dynamics’. Award winning essay in Science: http://www.sciencemag.org/content/336/6084/993.full?rss=1. Complete with open access interactive textbook: https://biol300.case.edu/wiki/index.php/Main_Page
- Carl on the impact of Science2.0
- Literature review.
- Development hiC in mouse and more ID of topological domains defined by insulators (Dixon 2012)
- Working on snail dose figures (see post). Sent to ML.
- Found bug in AutoZcal /write_min_dax_ini_file call — user defined inputs were being overwritten.
- substantially revised write_min_dax_ini_file to have more user defined inputs which are passed through the simple structure ‘pars’. e.g.: pars.min_height.
- Cleaned up routine previously covered by AutoZcal into core script AutoRun_beads_n_data.m, which calls subscripts: fxn_autoZcal (which calls fxnZcal and revised write_min_dax_ini_file), fxn_run2Dbeads, fxn_Visbead_alignment, fxn_IRbead_alignment, and fxn_insightM. These are bundled in subfolder movie2list. See github repo for more info.
- ML recombined paper themes. see if we can sort this out by email.
- troubleshooting error in revised auto-z-calibration script.