Monday 07/23/12

5:00P – 12:00 A

• Recomputing polyermase density for snail
• looking for twinned spots. Reasonably common. Half of cc13 embryo collection imaged is probably clean through replication of sna locus.
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  replicated_sna4b

  

  replicated_sna3b

  

  replicated_sna2b

  

  replicated_sna1b

  

  replicated_sna2

  

  replicated_sna1

• No G phases in early division (O’Farell 1994).  cc14 is the first to get a G2 phase.
• Replication persists for almost all of the cell cycle. Pericentric regions probably replicating last.  PCNA starts out disperse and concentrates into clusters over time.
• Revised mRNA degradation rate calculations to 5 min cell cycles (consistent with Thomas’ data and published results by O’Farell.  O’Farell says even 6 min mitosis coupled with 13 min interphase in cc13 (instead of 15min) .
• Revised synthesis rate calculations for DNA replication of snail locus in mid cc13.
• Running K4 analysis on Tuck and Cajal
• Literature review