9:50 A – 5:00 P, 9:30P – 10:45p
- Jiang lab meeting
- difficulty connecting to notebook
- 710 confocal all sessions canceled, new 780 being installed this week. Requested training on ELYRA which also has a 710 (and maybe a programable stage?) Planning Thursday to attempt imaging sequentially / by hand on 700.
- revising text for snail kinetics paper
- processing confocal images of last weeks’ in situs. Judging by 405 stain, rb 750 didn’t work. Also didn’t work on STORM images of H3K27-cntrl samples
- R&D anti-m antibody is designed for multi-label against rat and is preabsorbed against rat IgG.
- Ligations of 5 of the new probes templates transformed into bacteria.
- KE fixing embryos (Pc and yw) 2-9hrs.
- 4 new bottles of yws from O/N collection
- KE Testing Jackson rat-488 for background dots and rb-750 for function (labeling H3(?-confirm with KE)).
- Meeting with Ting and Xiaowei Wed.
- New fly crosses: Psc shRNA1 x 3x mat Gal4. Pc shRNA2 x 3x mat Gal4 appears to be lethal from early inspection, no crawling larvae visible yet but good number of eggs. Will re-inspect over next 10 days.