9:15 A – 7:00 P, 8:30 P – 11:20 P
- Finish O/N STORM run of Antp/Tub
- bead images
- z-calibration first iteration looks horrible. May need new few guess parameters. Attempting multiple iterations.
- simulating snail patterning with different promoters. Need substantial cell-cell coupling.
- Expand snail patterning simulation to whole snail pattern (prevents artifacts from edge effects.
- Polycomb knockdown results update:
- Pc-1 shRNA does not provide phenotype or lethality
- Pc-2 shRNA does not provide phenotype or lethality
- Psc-1 shRNA does not provide phenotype or lethality
- Clear cages (AM) and collect virgin 3x Mat Gal4 (9 PM). cages moved to 17C.
- Confocal imaging O/N of slide m105 on new Zeiss 780. scan speed 6, 3 channel imaging. red channel using Sh2 cooled enhanced efficiency detector.