9:30 A – 7:30P, 10:30 – 12:50A
- Break down O/N mp10a run.
- save mp07 data. All look good, 7 images finished completely, last image starts at the top of the mRNA field.
- etching S1 AbdA, AbdB, Ubx, K27me3.
- Issues booting STORM4. few reboots gets working (focus lock camera having trouble?)
- Realigned path to quadview to remove clipping effect. Discussion with Yari, probably a gradual relaxation effect due to slight tension in the bolted path.
- Bead imaging.
- Run analysis of mp10a. uniform 555 background looks pretty correctable. Calibrated sensitivity in green channel.
- Literature review:
- Weinberger (now USCF) reports pertubation response protein noise can distinguish birth/death noise vs promoter switching. Confirm prior claim that HIV noise is promoter switching driven.
- Pirrotta review (Science 2012 v337): suggests catalytic preference of PRC2/SuZ(12) for neighboring histones (and antagonized by K4 and K36 me3) provides the ‘global function’ of PRC2 in DI-methylating most of the genome that is not actively transcribed (rather than trimethylating PcG target genes).