9:20 A – 8:30, 9:30 – 11:10PM
- Flip collection cage
- take embryos out of 4C, final hour incubating at RT before washes.
- snail in situs Draq5 stained and mounted
- STORM / section in situs / DFISH all in secondaries at 4C O/N for greater penetration.
- Working on review article.
- Literature scan from rss feeds.
- Francis lab meeting
- see recent work by Bickmore lab on hox complex condensation (see Mendeley).
DNA FISH probe making
- new PolI DNAseI from Promega arrived. Creating Nick translation mix and calibrating with test DNA (BAC7).
- autoclaving glycerol to make new mix. (also autoclaving new LB)
- Running out first sample on gel.
- no sign of template DNA or smear of product DNA. Too much DNAse. Need more Pol1 and try again.
Starting cell culture experiments:
- Plate S2 cells, grow O/N in 8 well slides. Fix tomorrow for DNA centromere FISH
- Plate S2 cells, grow O/N in 8 well slides. Fix tomorrow for RNA FISH