Testing small oligopaint probes

First small test probe


  • The primer  has a 5′ TYE665 dye.
  • Both primers are at 200 µM (200 pmoles/µl).
  •  template was at 10 ng/µl.
A look at the genomic region
Zoom in on region


Question for Ting /Brian

Randomly ligate labeled primers with DNA probe fragments.  Way to use modified terminal nucleotides so that primers don’t ligate together.   HPLC to separate out the labeled labeled single strands.

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