Friday 12/14/12

10:15 A – 8:00 P, 9:00P – 11:30P

Probe making

  • LC library arrived today
  • IDT primers arrived

Cell culture

  • diluted recovering S2R+ culture and Kc160 cells (prior to splitting).
  • Move back to 28C incubator
  • Testing temperature in 28C incubator.
  • S2 cell new line established by NF.  Will be ready tomorrow.
  • dilute cells 1:2 and split into new small culture flasks.

Submitting Cell Reports revisions

  • Incorporate ML edits to main text and reviewer response (use Cajal Word to see the track changes)
  • convert supplemental material from TeX into Doc using Cajal.
  • Inventory of supplemental material.
  • submitted, 4:58PM
  • working on possible cover arrangements.  (To be submitted separately).

Working on lab meeting presentation

  • introduction: types of cell memory
  • basic mechanisms of Pc action / heterochromatin

# Antibodies

  • # Make glyercol stocks of:
  • Santa Cruz rb anti-H4K16ac
  • DSHB m anti-HP1a
  • DSHB m anti-Gro
  • DSHB m anti-Zw5
  • DSHB m anti-BEAF

 

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