9:40 A – 8:00P, 9:30-10:30P
- final prep for lab meeting
- Give lab meeting presentation 10A-1P
- Meeting with XZ, ~4P
- Meeting with Max, discuss MTD knockdown ~4P
Snail
- Missing JPA and highlights for CR paper, upload now
- Cell reports submission site seems to be down
- Now uploaded.
Antibodies
- Aliquot 60% of DSHB antibodies (Gro, BEAF, Zw5, Hp1) and santa cruz antibody (H4K16ac)
- Passage cells. PolyL coat coverglass. Treat cells.
- Fix cells to test antibodies.
Fly Work:
- Move F1 Pc1 shRNA females to 29C in fresh vial. (Reflip into fresh vials at 29C tomorrow as well). Screen vials for hatchlings.
- Continue expanding sim[D] and Espl[D] lines — growing quite slowly…
Probe making
- 1 umole primer dilute in 5mL for 200 uM primer stock for PCR labeling reactions.
- Resuspend original sample in 500 uL of DNAse free ddH2O and dilute 100:900 for working stock.
- No: final yield is actually only 169 nmoles of probe. Dilute in 845 ddH2O -> 200 uM stock.
- Dilute 784 nmoles of probe 3.92 mL for 200 uM biotin stock.