Monday 12/17/12

9:40 A – 8:00P, 9:30-10:30P

  • final prep for lab meeting
  • Give lab meeting presentation 10A-1P
  • Meeting with XZ, ~4P
  • Meeting with Max, discuss MTD knockdown ~4P

Snail

  • Missing JPA and highlights for CR paper, upload now
  • Cell reports submission site seems to be down
  • Now uploaded.

Antibodies

  • Aliquot 60% of DSHB antibodies (Gro, BEAF, Zw5, Hp1) and santa cruz antibody (H4K16ac)
  • Passage cells.  PolyL coat coverglass.  Treat cells.
  • Fix cells to test antibodies.

Fly Work:

  • Move F1 Pc1 shRNA females to 29C in fresh vial.  (Reflip into fresh vials at 29C tomorrow as well).  Screen vials for hatchlings.
  • Continue expanding sim[D] and Espl[D] lines — growing quite slowly…

Probe making

  • 1 umole primer dilute in 5mL for 200 uM primer stock for PCR labeling reactions.  
  • Resuspend original sample in 500 uL of DNAse free ddH2O and dilute 100:900 for working stock.
  • No: final yield is actually only 169 nmoles of probe.  Dilute in 845 ddH2O -> 200 uM stock.
  • Dilute 784 nmoles of probe 3.92 mL for 200 uM biotin stock.

 

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