Thursday 12/20/12

1:45P – 5:00P

  • Move cells to LN2
  • LN2 in Jr Dewar below visibly detectable level (empty?)
  • Refilled Dewar, informed Alec/Eileen/Jiang about issue.
  • Sensor was reading 1/2 steadily.  Now reading full, then alarm goes off and it reads empty for a while, and returns to full upon press of alarm acknowledge.

Fly work

  • check Pc shRNA vials at 29C — still laying healthy developing embryos / hatching fine.
  • Flip sim[D] and Espl[D] vials into bottles.

Confocal

  • check embryo test of new antibodies
  • images still on Zeiss780.
    • Zw5 not work well, little detectable staining
    • BEAF clear foci in nuclei (probably sparse)
    • HP1 pretty dense foci in nuclei.  Wonder if these are clusters.  Not much of the obvious spatial organization present in the S2 cells.
    • abcam H3K9me2 looks pretty good, bright strong and punctate clusters
    • rb H4K16ac 488 — not  detectable, though looked good in S2 cells.  Maybe it’s the glycerol, or the concentration is too low for embryos (Sc ships antibodies and 10x dilution relative to abcam…. (.2 mg/mL).  Should also test in Cy3B / brighter conventional dye.
    • [spreadsheet 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 602 250 sheet=6]
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