Researching UAS-LacZ line sequences to probe stochastic expression effects.
my UAS-LacZII from flybase stock 3955,
From Kathy Matthews, 1998 gift of flybase.
contains: P{UAS-NZ}20b — SV40 T NLS-lacZ fusion in pUAST (beta-gal is localized to the nucleus). Insertion on chromosome 2. From Yash Hiromi and S. West, unpublished.
LacZ on III BSC 3956, is from same collection: P{UAS-NZ}J312 — Insertion on chromosome 3. From Yash Hiromi and S. West, unpublished.
comes from progenitor pUAST. Map:
has 5x UAS-sites.
Reference: Brand and Perrimon 1993
Report comment: Contains 5 tandemly arrayed, optimized GAL4 binding sites (UAS\’s), followed by an Hsp70 TATA box and transcriptional start, a polylinker with 6 unique sites, and the SV40 small-t intron and polyA site.
Maybe it’s the TATA that makes the expression bursting instead. Need to do a Drosophila promoter TATA box exploration.