10:00 A
- Group meeting (Eileen) see notes
- Journal club: High salt recruits aversive taste pathways
STORM
- Hal crashed on STORM3 at 10:11A (still 34 locations acquired).
- sent STORM3 error report to Hazen (see email record)
- STORM2 run seemed to finish without error.
- Set up O/N run on STORM3 to do 2x-cy5 (previously imaged on STORM2).
- Set up O/N run on STORM2 to do 3x-cy5 (second biological replicate).
Probe Design
- Jeff proposes exonuclease approach to test
- I miscalculated original probe. A 10mL scale reaction uses 100 mL of 100 uM primer. That’s 10.0 nmols of primer, not 100. nmols. So a yield of 0.5- 4 is reasonable, and there’s much less room to improve than estimated.
- Brian has 405 primer with 20 bp that I can use for the chromatin colors library.
- new design layout
Coding
- Need to remove L1 Homotopy from Beta folder
- Previous External did not remove after first jeff branch.
- So the short-version I applied was incomplete because of the unmatched files. This more complete command from the git help completely gets rid of things. I checked every entry this time, not just the first ~20 and last 5 (which was stupid because we didn’t move Insight and DaoSTORM into the repo until after the first few commits.
Correct approach:
git filter-branch --force --index-filter 'git rm -rf --cached --ignore-unmatch ./External' --prune-empty --tag-name-filter cat -- --all
rm -rf .git/refs/original/
rm -rf .git-rewrite
- coding directory now properly updated, synched and merged.
- TdG will get back to us in a few weeks about beta tests of STORMrenderBeta