11:15 A – 8:15 P, 9:30 P-11:55 P
Review
- working on explanation of individual site contributions to cooperativity: Jiang 1993
- Lebrecht et al 2005 make Bcd rescue embryos lacking the Bcd interaction domain required for cooperativity in vitro.
- They observe a dramatic degree of variation in the levels of hb expression in anterior regions. This is consistent with the prediction of high-noise sensitivity of cooperative systems.
- They observe very little change in boundary of expression. This is not readily consistent with the expectation of cooperative systems, except that perhaps the difference between 6 fully cooperative sites and 1 site is such that the levels of Bcd needed to ensure complete occupancy without cooperativity are not obtained in the embryo.
- It is likely that some degree of feedback is also needed to explain these results, such as the known hb, bcd cooperativity (Triesman1993)
Reading
- Affymetrix files patent on oligopaint-like protocol
STORM
- imaging ph-Mutant
- destroyed ph-Mutant conA slide: mounted upside down. 🙁
- O/N run of ph-Mutant (very low density). Imaging some non-transformants as background control.