Structural and molecular interrogation of intact biological systems

CLARITY hydrogel electrophoretic tissue transmutation.

Nature Paper

  • cross link gel. Add ionic detergent (SDS micelle), extract all membranes in electrophoresis
  • tissue becomes much clearer, can image deep
  • proteins still there.
  • do they demonstrate nucleic acids? – doesn’t look like it.
  • how about charged proteins? Retained by gel?
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