Saturday 04/20/13

11:00 A – 5:00 P, 7:30 P – 11:50 P

Project 2 updates — see post

Talk outline

• Introduction
  1. Lots of regulatory DNA
     a. ENCODE
     b. Drosophila enhancer trap.
     c. Stark lab screen
  2. Complex organization of enhancers (binding sites)
  3. Goals of modeling
     a. effect on expression levels
     b. expression sensitivity
     c. expression noise
  4. Experimental measures
     a. expression levels
     b. expression dose sensitivity (mRNA vs. Bcd). Bcd binding vs Bcd?
     c. expression noise
     d. transcription kinetics
• Outline/Themes/Goals transition
• Types of models?
  1. deterministic, chemical master equation, finite Makov chain

Observations

• Texmaths plugin for LibreOffice4 is awesome.

Fly Work

• Flip Fly Stocks
• Flip all fly bottles. Downsize to one bottle per line.