9:00 A – 7:00 P, 8:40 P – 12:20 A
check concentrations
Measured in ug total DNA
| Sample | PCR ng/uL | Filtration | EtOH | EtOH no glyc | Nicking |
|---|---|---|---|---|---|
| #1 Y nick | 41.8 | N/A | 36 | 37 | 10 |
| #2 B nick | 26.4 | N/A | 21 | 14 | 7 |
| #3 ubx nick | 24.1 | N/A | 39 | 0* | 10 |
| #4 Y PPT | 21.8 | 7.95 | 25.3 | 14 | N/A |
| #5 Y CPPT | 26.6 | 8.85 | 31 | 13 | N/A |
Time series digestion measured in ug/mL
| Sample | T=0 | T=1.5 hrs | T = 10 hrs |
|---|---|---|---|
| #4 Y PPT | 38 | 13 | 13 |
| #5 Y CPPT | 15 | 7 | 8 |
Fixing gels:
- Try running denaturing gels immersed in 50C waterbath. Monitor temperature with electronic thermometer.
- Test gel. Lane order:
| #1 PCR | #2 PCR | #3 PCR | #4 PCR | #1 final | #2 final | #3 final | ULR 1:10 | T7 #4 | T7 #5 | y1 DE | y10 DE | B10 DE | #6 y assym| 5 uL ULR
STORM
- setup O/N STORM of S2 cells (Ph negative control).
- look pretty clean, some clustering of background.
newstart = 1;
setmessage = codemessage;
while newstart
Other trouble-shootin
- Remember to pre-equilbrate DNA clean-up columns