9:15 A – 12:15 A
STORM
- Finish STORM O/N run
OligoPaint probe synthesis
- Ubx on 50C denaturing gel looks decent. nice tight band large band after PCR, splits into two bands after nicking. Unfortunately most of the product is not nicked.
- y runs as a smear
To run on denaturing test gel:
* #7, #8, #9,
Ubx after filteration column?
Gel Extraction
- Ubx and Y1 mL
- NO DNA?! Overfilled buffer – DNA flowed out of top? extra EtOH?
Transcription Modeling
- Write and email abstract for Chicago talk!
- Work on manuscript revisions