9:30 A – 7:00 P, 9:35 P – 11:15 P
Post-doc candidate interview
- see notes
- Gheorghe Chistol (Bustamante Lab)
Presentation stuff
- Touch up slides for post-doc candidate chats
Communication
- Feedback from Chicago: Some support from Stats department
- Ecology/Evolution finds “math too simple” — probably over emphasized simplicity
- updated CV and website with recent presentations and publications
- Sent CV to John.
OligoPaints
- O/N 37C evaporates all ~144 uL of H2O for resuspending DNA pellet
- resuspended DNA samples separate out into cloudy non-soluble fraction. Hopefully this is just all the protein
- Precipitating 5 mL scale Y and K DNA
- Resuspending Y and K DNA
- Testing 1:20 dilutions on Agarose gel compared to original PCR at 1:10 dilutions and ladder at 1:10 dilution
- concentrations all look low. Maybe recovery from 50 mL falcon’s not so good.
Bead based extraction prep
- Order regents
- PEG 8000 (soluble up to 63% in ddH2O, better in warm water 80-90C).
- ThermoScientific magnetic beads, 15 mL
Coding
- Finish writing SteveViewer
- lost multicolor support
- restored multi-mag support. Automatically downscales to save memory