Monday 07/01/13

9:45 A – 8:00 P 9:00 P – 12:00 A

STORM

  • O/N STORM lost focus early in night :(. Reset focus, got a few more cells during 4.5 hour lab meeting.
  • Replaced buffer on chiseled PhF slide. Setting up to image O/N.

chromatin project

  • received 10kb size HiC data from Li Li.
    HiC_domains

HiCdomains_w200kb_flanks

  • Script failed to execute all 40 probe regions (stopped at 9 had small bug in code). Now designing probes for remaining regions.

Shu project

  • stain embryos for deep imaging with Shu tomorrow.

limb bud

  • received word from Aysu, plan discussion meeting sometime this week.
  • starting to record sequences at 4C contacted enhancers in limb bud. Should also get intervening sequences.
  • saved in TargetRegions.ods, sheet3 (should move to online).
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