chromatin paints activator tests

Possible matches:
* X-1, cy5-2
* 0-1, cy5-2
* 405-1, cy5-2
* cy5-1, cy5-2
* cy5-1, X-2

Blind-Analysis

Round 0

Round 1

  • R1S1 0-1, cy5-2
  • R1S3 405-1, cy5-2
  • R1S4 cy5-1, X-2
  • R1S5 cy5-1, cy5-2
  • R1S6 X-1, cy5-2

Round 2

  • R2S1 guess = 0-1, cy5-2
  • R2D guess = 405-1, cy5-2 (rpt 405?)
  • R2F guess = cy5-1, cy5-2
  • R2B guess = 0-1, cy5-2 (rpt 2-only?)
  • R2S7 guess = cy5-1, 0-2? (Fewest localiztions — ~75% of dots don’t make the 300 cutoff)
  • R2S2 guess = 405-1, cy5-2 (rpt 405?)
  • R2S1 guess = 0-1, cy5-2 (rpt 2-only?)

Counting complete for

  • R2S1
  • R2D
  • R2F

Lessons from this annotation

  • Adventage of auto then screen is no time lost waiting for data to load and process, can just click through
  • need to fix export of Overview image
  • should add pause to auto so that files for a common locus are saved sequentially in time. (Or lead with name?)

Different classes of structures

  • sometimes in stretched or even looped/bent configuration.
  • most prevalent is the compact configuration.
  • density still always high.

storm_R2S2_im21_dot02

storm_R2S2_im21_dot01

storm_R2S2_im21_dot04

This entry was posted in Chromatin. Bookmark the permalink.