(Sebastian presenting)
Paper
- Lang … Chin Nature chemistry 2012.
- currently method of choice in the field.
background
- site specific labeling of single cystine. Hard to remove extra Cys
- Fluorescent protein fusions (large, may have major functional impacts).
- self-labeling proteins: SNAPtag, HALOtag, CLIPtag: small enzymatic proteins attach the fluorphore.
- Ting lab / biotin ligase, lipolic acid ligase etc.
- all have to introduce a some substantial protein sequence into the target of interest.
- Alternate approach: encorporate genetically encoded unnatural amino acids. Use these for bio-orthogonal reaction chemistry. (draw-back: slow reactions ~30