- Bauer Core equipment training
option 1
Amplify sublibraries
* Buy NEB DNA kit, end repair
* blunt end ligation
alternative
- PCR primer
- gel-extraction
- Qubit concentration (nanodrop?) [optional]
- BioAnalyzer at Bauer Core. [optional]
- qPCR, dilute sample a few times. By Illumina qPCR kit from kappa.
- Mx3000p (less used) qPCR machine at Bauer core. On every sublibrary with my index.
- use this concentration to dilute to 10 nM single strand. Combine and submit.
- (triple measure of concentration).
- low complexity sequence problem for reader.
- which primer gets read from.